• br Design of donor plasmids If no footprintless

  2018-10-24

  

  Design of donor plasmids If no footprintless gene targeting is required or it is intended to introduce larger transgenes, donor plasmids have to be constructed that contain the homology arms flanking the transgenes or selection cassettes coupled to promoters of choice. Similarly, donor plasmids c

• Here we started to address to what extent GFAP ventricular

  2018-10-24

  

  Here, we started to address to what extent GFAP+ ventricular cells, denoted as ependymoglia cells (Parish et al., 2007), within and outside of the constitutively active niches are different from each other in the newt telencephalon. We define two different types of ependymoglia cells, which display

• Recently several clinical trials of stem cell based therapy

  2018-10-24

  

  Recently, several clinical trials of stem-cell-based therapy for SCI using either human NS/PCs (Cummings et al., 2005; Salazar et al., 2010) or human ESC-derived OPCs (Strauss, 2010) have been initiated. Compared with these stem cells, iPSCs raise fewer ethical concerns in certain countries (Nori et

• In the controversial field of

  2018-10-24

  

  In the controversial field of pluripotent stem cell research, it is vital to argue on the basis of reliable and solid data that best reflect the actual research situation and are carefully validated. However, available data on recent research activities in this field are often based on abstract sear

• br Results To develop iPSC

  2018-10-24

  

  Results To develop iPSC-based model systems of disease, we first prepared a bank of >60 iPSC clones (ten clones per donor; partial set and reprogramming methodology described previously [Mills et al., 2013; Somers et al., 2010]) derived from the dermal fibroblasts of three control individuals wit

• br Funding This work was funded by NIHR GM awarded

  2018-10-24

  

  Funding This work was funded by NIHR01GM085456 awarded to Tanja Dominko. Declaration of interest The following are the supplementary data related to this article. Acknowledgements Introduction The identification of culture conditions that promote the expansion of primitive hematopoieti

• The NT formation and NESC to RGPCs transition

  2018-10-24

  

  The NT formation and “NESC-to-RGPCs” transition at the onset of neurogenesis are two fundamental events in the early developing AMG 925 (Götz and Huttner, 2005). However, the developmental regulations are unclear due to being hard to definitely control the two stages. In this study, the two stages

• To elucidate whether the expression

  2018-10-24

  

  To elucidate whether the expression pattern of Zscan4 has any correlation with ESC proliferation, we monitored Zscan4 activity at single cell level. Also to see whether the rejuvenation factor Zscan4 correlates with the fluctuating wave of ESC pluripotency (Figure S1), we monitored Zscan4 and the pl

• br Experimental Procedures br Author Contributions br

  2018-10-24

  

  Experimental Procedures Author Contributions Acknowledgments Our thanks go to Gioia Polidori Francisco for training and discussions, Kate Watt and Yvonne Turnbull for technical and laboratory managerial support, Kadri Oras and Laura Ferguson for experimental support, Po-Lin So and Bruce Con

• Traditionally testing of hPSC lines for gross chromosomal ch

  2018-10-24

  

  Traditionally, testing of hPSC lines for gross chromosomal changes employed karyology by chromosome banding of metaphase aldose reductase inhibitors (Amps et al., 2011; Baker et al., 2007). Although karyotyping allows examination of the entire cell genome in a single assay, an often overlooked issu

• FHPI One major drawback of current melanoma therapy is the

  2018-10-24

  

  One major drawback of current melanoma therapy is the development of resistance mechanisms against novel therapeutics targeting the MAPK members RAF or MEK. Upon MAPK inhibition, additionally acquired mutations reactivate the MAPK pathway by gain of RAF gene copy numbers (Shi et al., 2012; Villanuev

• br Results br Discussion Our data indicated

  2018-10-24

  

  Results Discussion Our data indicated that phenotype-linked transcriptomics of GSCs overlapped with tumor anatomic site, with mesenchymal-like/nodular signatures prevalent in perinecrotic zones and proneural-like/invasive signature in infiltrating areas of tumor, suggesting that these GSCs bot

• Aside from yield point change structural element

  2018-10-24

  

  Aside from yield point change, structural fdps refinement can also affect the melting temperature. The latter is determined experimentally for micro- and nanodisperse materials, but not as definitively as the yield point. For example, aggregate and powder metallic nanomaterials may have different m

• The present work reports for the first time the synthesis

  2018-10-24

  

  The present work reports for the first time the synthesis of Ca5(PO4)3Cl:Eu2+ phosphor via the Pechini (citrate gel) method and the investigation of its photoluminescent properties. Experimental For synthesis of Eu2+ activated chlorapatite phosphor Ca5(PO4)3Cl stoichiometric amount of the precur

• br The subject of the study materials

  2018-10-24

  

  The subject of the study, materials and experimental procedures The experiments were carried out at the T.I. Vyazemsky Karadag scientific station – Nature Reserve of RAS (Feodosia) in an enclosed concrete 27m×9m×4.5 m-sized pool. An adult Black Sea bottlenose dolphin (Tursiops truncatus) who had

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