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Our results also appear to be in
2018-11-08
Our results also appear to be in line with earlier observations that ABCG2 expressing cells are overall smaller than cells positive for CK3 (de Paiva et al., 2006; Kim et al., 2004). As to the effect of oxygen, it is interesting to note that both the ABCG2+ and CK3+ were in general larger in sub-amb
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One of the main obstacles in human studies
2018-11-08
One of the main obstacles in human studies is sample acquisition. Human iPSC lines are usually produced from fibroblasts isolated from skin biopsies, a traumatic invasive procedure demanding local anesthesia and medical follow-up. Hair follicle (HF) plucking, however, is simple, painless and non-inv
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Cilengitide manufacturer TGF type I receptor inhibitors were
2018-11-08
TGF-β type I receptor inhibitors were also found to induce differentiation of insulin-producing cells. Among the TGF-β type I receptor inhibitors that we tested, Alk5 inhibitor II showed unique effects on the differentiation of pancreatic endocrine cells. Only Alk5 inhibitor II significantly increas
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In our model DNA damage forces cells
2018-11-08
In our model, DNA damage forces cells into cellular senescence, whereas ATM-dependent and p53-antagonized cytokine secretion activates BMP2/JAK-STAT signaling and stimulates the differentiation process in a progressive feed-forward manner. This senescent state is very different from the GFAP-associa
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br Experimental Procedures br Acknowledgments This work was
2018-11-08
Experimental Procedures Acknowledgments This work was supported by BONFOR/Gerok Scholarships O-137.0015 (to B.V.S.), Ruediger Foundation grants (to B.V.S.), State Scholarship Fund/Chinese Scholarship Council 2008627116 (to Z.L.), NIH – National Eye Institute 5R01EY022079-02 (to S.T.), and NYSD
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In RPE transplantation it is widely accepted that grafted ce
2018-11-08
In RPE transplantation, it is widely accepted that grafted Merimepodib perform better in terms of their morphology, physiology, and survival when transplanted as sheets rather than in suspension. It appears that environmental factors provided by cell sheets, such as extracellular matrix and adhesio
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ephrin receptor br Materials and methods br
2018-11-08
Materials and methods Results Discussion Using a dual-promoter lentivirus-transduced BM-HSC transplantation model, we report here that both transgenes (i.e. murine invariant chain (Ii) and the GFP reporter) can be detected in various APC cell types from different organs (Fig. 1). Thus, this
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Gene expression is regulated by complex coordinated processe
2018-11-08
Gene expression is regulated by complex coordinated processes, including chromatin remodeling, post-translational modifications of histones, and incorporation of non-allelic histone variants. The histone variant H2AZ constitutes only a few percents of the total H2A cellular pool (). However, H2AZ is
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Our previous findings in the pelvis of mice from
2018-11-08
Our previous findings in the melanotropin of mice from the same experiment already indicated significant bone loss and alterations in the structural architecture of the ischium, likely due to reduced anti-gravity and posture-maintenance muscle–bone interaction forces (Blaber et al., 2013). In contr
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br Mesenchymal stem cells The concept of cooperation
2018-11-08
Mesenchymal stem cells The concept of cooperation between stem 5 aminolevulinic acid and cardiomyocytes, functioning as supporting cells within the myocardial niches, has been documented carefully. The interaction between exogenously delivered bone marrow MSCs and endogenous c-kit-positive CSCs f
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br Cardiac stem cell niches A challenging
2018-11-08
Cardiac stem cell niches: A challenging task The importance of the niche microenvironment for understanding HSC behavior was acknowledged shortly after the discovery of this adult stem cell class. In the absence of niches, the stemness of the primitive buy Tunicamycin cannot be preserved. Outside
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atp gamma s manufacturer Additionally it would be highly des
2018-11-08
Additionally, it would be highly desirable to manipulate CSCs to improve their survival and direct them efficiently to the myocyte lineage. A recently developed technology to deliver mRNA in vivo shows promise. Modified RNA (modRNA) encoding human vascular endothelial growth factor-A (VEGF-A) improv
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From an analytical perspective we
2018-11-08
From an analytical perspective, we applied the approach that combines application of high pressure assisted protein extraction and a combination of two LC/MS/MS techniques: electrospray ionization (ESI)-MSe and MALDI-TOF/TOF (Mindaye et al., 2013a, 2013b). Label-free quantification of proteins was p
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Keeping SCs out of the
2018-11-08
Keeping SCs out of the active nobiletin phase and minimizing the risk of DNA damage may be especially important in aging and has led to a SC hypothesis of aging (Sharpless and DePinho, 2007; Beausejour and Campisi, 2006). We succeeded in transiently inhibiting DREAM expression 24h after siRNA trans
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The present results demonstrate that GABP is important
2018-11-08
The present results demonstrate that GABP is important for myeloid differentiation confirming the recently reported observations by us and others in hematopoietic cell lines and murine models, respectively (Yang et al., 2011; Yu et al., 2011; Ripperger et al., 2015). Here, for the first time, human
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